Mutation hcaD787(del)::kan
- Name: hcaD787(del)::kan
- Type: Insertion
- Mutation of Gene: hcaD
- Approx. Map Location: 57.55
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were GCGGCATTCGCCACGTAGCGGCTGGGCTGGATCTTTAAGGAAGCACGATGATTCCGGGGATCCGTCGACC and GGCATTGAGATATTCGCTAATTATCCTGGTTACAGTGATTTAAGCGCGATTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW2526-2 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, ΔhcaD787::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514