Mutation hemX750(del)::kan
- Name: hemX750(del)::kan
- Type: Insertion
- Mutation of Gene: hemX
- Approx. Map Location: 85.91
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were ATGCGCTTTTACGGGCATTACAATAACTCTCATAACAGGAAGCCATAATGATTCCGGGGATCCGTCGACC and CACAAAGAGCAATAACACTTTTAGCATGGTTATTCTCCTTGCGGCGCGGCTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW3775-2 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, rph-1, ΔhemX750::kan, Δ(rhaD-rhaB)568, hsdR514