Mutation hsdR748(del)::kan
- Name: hsdR748(del)::kan
- Type: Insertion
- Mutation of Gene: hsdR
- Approx. Map Location: 98.74
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were GGCAGCCTGAAGGATGAAGTGTATACGTGTCAGGCCAGCTCGTCCCAGATATTCCGGGGATCCGTCGACC and CTGTGACCGTGGTCATTGCCCGGAAAGGTACAGAAAGCTAAGATGAGATGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW4313-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514, ΔhsdR748::kan