Mutation iscR777(del)::kan
- Name: iscR777(del)::kan
- Type: Insertion
- Mutation of Gene: iscR
- Approx. Map Location: 57.33
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were CACTCCGGCCTGATTCTGAATTCTTTTTATTAAGCGCGTAACTTAACGTCATTCCGGGGATCCGTCGACC and TACAATAAAAAACCCCGGGCAGGGGCGAGTTTGAGGTGAAGTAAGACATGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW2515-3 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, ΔiscR777::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514