Mutation leuA781(del)::kan
- Name: leuA781(del)::kan
- Type: Insertion
- Mutation of Gene: leuA
- Approx. Map Location: 1.77
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were CAATACGGCAATATGGTAATTCTTCGACATCACACGGTTTCCTTGTTGTTATTCCGGGGATCCGTCGACC and TTTTATGCCCGAAGCGAGGCGCTCTAAAAGAGACAAGGACCCAAACCATGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW0073-1 7 F-, Δ(araD-araB)567, ΔleuA781::kan, ΔlacZ4787(::rrnB-3), λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514