Mutation leuD778(del)::kan
- Name: leuD778(del)::kan
- Type: Insertion
- Mutation of Gene: leuD
- Approx. Map Location: 1.70
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were AAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGATTCCGGGGATCCGTCGACC and GACCGGACATTTCGCCGACATTCGCAACATTAAATAAGGAGCACACCATGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW0070-1 7 F-, Δ(araD-araB)567, ΔleuD778::kan, ΔlacZ4787(::rrnB-3), λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514