Mutation melB746(del)::kan
- Name: melB746(del)::kan
- Type: Insertion
- Mutation of Gene: melB
- Approx. Map Location: 93.57
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were GAAGGCGGGCGTTGGTAACAGCGACCCGATACCCTATGAGCATTTCAATGATTCCGGGGATCCGTCGACC and CAGGCCATTTTCGTTAGCTATTGTCCACGTCAGGCTTTCACATCACTCACTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW4081-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, rph-1, Δ(rhaD-rhaB)568, ΔmelB746::kan, hsdR514