Mutation nagA726(del)::kan
- Name: nagA726(del)::kan
- Type: Insertion
- Mutation of Gene: nagA
- Approx. Map Location: 15.11
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were AGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTATTCCGGGGATCCGTCGACC and CCCTGGCTCCTTGCTCAGGGCAATATTTTTTAAAATCGGGGGTCAGAATGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW0663-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), ΔnagA726::kan, λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514