Mutation pgpA777(del)::kan
- Name: pgpA777(del)::kan
- Type: Insertion
- Mutation of Gene: pgpA
- Approx. Map Location: 9.48
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were TTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGATTCCGGGGATCCGTCGACC and CAAGCGTCACATCAGGCATCGGTGCACAACTACGACAGAATACCCAGCGGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW0408-4 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), ΔpgpA777::kan, λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514