Mutation rph-749(del)::kan
- Name: rph-749(del)::kan
- Type: Insertion
- Mutation of Gene: rph
- Approx. Map Location: 82.20
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were GTCCGTATAATGCGCAGCCACATTTGTTTCAAGCCGGAGATTTCAATATGATTCCGGGGATCCGTCGACC and TCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW3618-2 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, Δrph-749::kan, Δ(rhaD-rhaB)568, hsdR514