Mutation sufB758(del)::kan
- Name: sufB758(del)::kan
- Type: Insertion
- Mutation of Gene: sufB
- Approx. Map Location: 37.95
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were AAATCTTTAATACTTAACATGTTTATTCCTTATCCGACGCTGTGTTCAAGATTCCGGGGATCCGTCGACC and GGACTTAATCAGATATTCAAATTTCACAACCCTAAAGCCCAGAATGAATGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW5273-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, ΔsufB758::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514