Mutation yagK720(del)::kan
- Name: yagK720(del)::kan
- Type: Insertion
- Mutation of Gene: yagK
- Approx. Map Location: 6.28
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were GCCTTCTCACTGAGAAGGCCATTACCGACTTACAGTTCGATTTGGCTACAATTCCGGGGATCCGTCGACC and CCTGTACGGTCTGCTATAGACCGTAGGACCAAATACAGGTATATATTATGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW0271-1 7 F-, Δ(araD-araB)567, ΔyagK720::kan, ΔlacZ4787(::rrnB-3), λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514