Mutation yagR728(del)::kan
- Name: yagR728(del)::kan
- Type: Insertion
- Mutation of Gene: yagR
- Approx. Map Location: 6.42
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were TCAAAAAGCGGGTATGACATTGTTCCTCCTTAAACCACATCCGGCAGCTTATTCCGGGGATCCGTCGACC and GCGAAGCGAACGCTTGCCTCCGTACTGGCTGAAGCGAGGGCACAGGCATGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW0278-2 7 F-, Δ(araD-araB)567, ΔyagR728::kan, ΔlacZ4787(::rrnB-3), λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514