Mutation yceD755(del)::kan
- Name: yceD755(del)::kan
- Type: Insertion
- Mutation of Gene: yceD
- Approx. Map Location: 24.70
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were CCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGATTCCGGGGATCCGTCGACC and TTTATCCCCGATCCAACGGGGAGCACCAATTACTTACGCTTTAAGCTGGCTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW1074-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, ΔyceD755::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514