Mutation ycfP770(del)::kan
- Name: ycfP770(del)::kan
- Type: Insertion
- Mutation of Gene: ycfP
- Approx. Map Location: 25.10
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were GGACTCGGCTCGCTGGAAAGCGATCAGCACCCGTCTGAATCAGTTACATGATTCCGGGGATCCGTCGACC and GGCAAGGTTACCTGATGCGACGAGGGCATTTACCCGAGGGTTTTGAACGCTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW5158-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, ΔycfP770::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514