Mutation ycjF759(del)::kan
- Name: ycjF759(del)::kan
- Type: Insertion
- Mutation of Gene: ycjF
- Approx. Map Location: 29.82
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were ATTCGTCTTGATGCTGCGCTGGAATTTTTAATAGGAGATAAATTGCGATGATTCCGGGGATCCGTCGACC and AAGATATCCGGTCAGCACCCGATATTGCATTATTTTTCGCTGGGCGTTTTTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW1315-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, ΔycjF759::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514