Mutation yecN723(del)::kan
- Name: yecN723(del)::kan
- Type: Insertion
- Mutation of Gene: yecN
- Approx. Map Location: 42.04
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were GTGGCATCAGACCACTACGCCTTATCTTTTTTTACATACGCCAGACATTGATTCCGGGGATCCGTCGACC and CCGGGAAAAAGAAAGTGGCGTATTGTGCGCTAACGCAGGGAGAAAACCAATGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW5308-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, ΔyecN723::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514