Mutation yfhK730(del)::kan
- Name: yfhK730(del)::kan
- Type: Insertion
- Mutation of Gene: yfhK
- Approx. Map Location: 57.93
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were AAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGAATTCCGGGGATCCGTCGACC and CCTTGAAACGCTGGCCCGTTTTTCCCCGCTCATTACGACAACTGGTAATGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW5407-2 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, ΔyfhK730::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514