Mutation yggL734(del)::kan
- Name: yggL734(del)::kan
- Type: Insertion
- Mutation of Gene: yggL
- Approx. Map Location: 66.81
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were TTTGCATTTGTCATCGCCCGTATGCTTTCTTAGTCCCACCAAACGTCGAAATTCCGGGGATCCGTCGACC and CGTCTTGGTCACGGAGTATGGGACTTAATGTTCGAGAGGGTGAAATAATGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW2926-2 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, ΔyggL734::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514