Mutation ymfE726(del)::kan
- Name: ymfE726(del)::kan
- Type: Insertion
- Mutation of Gene: ymfE
- Approx. Map Location: 25.79
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were TGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTATTCCGGGGATCCGTCGACC and TATATAATGACACTTAATTAAATATTACCATTACACTATCCTAAAGCGTGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW5166-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, ΔymfE726::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514