Mutation - efeU'-763(del)::kan

Mutation - efeU'-763(del)::kanMutation efeU'-763(del)::kan

Mutation efeU'-763(del)::kan

Name: efeU'-763(del)::kan
Type: Insertion
Mutation of Gene: efeU'
Approx. Map Location: 23.29
Properties
Property Symbol
deletion del
kanamycin resistant
Comments
- efeU' is not a functional gene in E. coli K-12 because of a fraameshift mutation. The wildtype gene encodes a membrane protein of the EfeUOB Fur-dependent inducible low-pH ferrous iron transporter.
- The primers used to make this knockout were ATTGTCAGTTTGATTGCCAGCTATCTTAAGCGTACCCAGCGAGGCCGATGATTCCGGGGATCCGTCGACC and TAACTAAGCAAGAGTTTGCGTCGTATTTGTTACGCGGAGCGAGACGCTGTTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
References
- Baba, T., T. Ara, M. Hasegawa, Y. Takai, Y. Okumura, M. Baba, K.A. Datsenko, M. Tomita, B.L. Wanner, H. Mori 2006. Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2:1-11
- Grosse, C., J. Scherer, D. Koch, M. Otto, N. Taudte, G. Grass 2006. A new ferrous iron-uptake transporter, EfeU (YcdN), from Escherichia coli. Mol.Microbiol. 62:120-131
1 Strains Carrying This Mutation
Name Mutations Genotype
JW1002-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ^-, ΔefeU'-763::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514

Property	Symbol
deletion	del
kanamycin resistant