Mutation fixA754(del)::kan
- Name: fixA754(del)::kan
- Type: Insertion
- Mutation of Gene: fixA
- Approx. Map Location: 0.91
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were ACACCACCGATATGAACGACGTTTCCTTCATGATTTCTGGAGATGCAATGATTCCGGGGATCCGTCGACC and AGAAAACGTGTTCATAGCATCCCCTGTAATTAAATGACTTTGCGAAGATTTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW0040-1 7 F-, ΔfixA754::kan, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514