Mutation gidA770(del)::kan
- Name: gidA770(del)::kan
- Type: Insertion
- Mutation of Gene: gidA
- Approx. Map Location: 84.53
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were CCCGGGCTTCAATCCATTTTCATACCGCGTTATGCGAGGCAATCACCATGATTCCGGGGATCCGTCGACC and GCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW3719-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, rph-1, ΔgidA770::kan, Δ(rhaD-rhaB)568, hsdR514