Mutation ygjI768(del)::kan
- Name: ygjI768(del)::kan
- Type: Insertion
- Mutation of Gene: ygjI
- Approx. Map Location: 69.49
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were AAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGATTCCGGGGATCCGTCGACC and AAATGCCTGCTCATTTTTTATTATTCTGCTTAATCAACATTGCTGGCTGGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW5512-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, ΔygjI768::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514