Mutation - polA12(ts)

Mutation - polA12(ts)Mutation polA12(ts)

Mutation polA12(ts)

Name: polA12(ts)
Type: Unknown
Mutation of Gene: polA
Approx. Map Location: 87.18
Properties
Property Symbol
Temperature Sensitive ts
Comments
- : sensitive to temperature and to low salt; altered pH optimum. Normal Exo levels. Totally defective in nick translation at 42C. recA56 polA12 are ts for growth.
References
- Uyemura, D, DC Eichler, IR Lehman 1976. Biochemical characterization of mutant forms of DNA polymerase I from Escherichia coli. II. The polAex1 mutation. J. Biol. Chem. 251(13):4085-9.
- Grindley, ND, WS Kelley 1976. Effects of different alleles of the E. coli K12 pol A gene on the replication of non-transferring plasmids. Mol. Gen. Genet. 143(3):311-8.

Property	Symbol
Temperature Sensitive	ts

10 Strains Carrying This Mutation

Name	Mutations	Genotype
ES1	4	F-, lacY1 or lacZ53(Am), uvrC34, rpsL31(strR) or rpsL151(strR), polA12(ts)
AB6007	7	F-, lacY1, uvrC34, thyA50, rpsL31(strR), rpsL151(strR), polA12(ts), deoC8
ES2	7	F-, lacZ53(Am), λ^-, IN(rrnD-rrnE)1, rpsL151(strR), polA12(ts), rha-5, uvrA6
MM386	7	F-, lacZ53(Am), λ^-, relA1?, rpsL151(strR), polA12(ts), rha-5, deoC2?
MM383	8	F-, lacZ53(Am), λ^-, thyA36, IN(rrnD-rrnE)1, rpsL151(strR), polA12(ts), rha-5, deoC2
MM387	8	F-, lacZ53(Am), λ^-, relA1?, recB21, rpsL151(strR), polA12(ts), rha-5, deoC2
MM385	9	F-, lacZ53(Am), λ^-, recA56, relA1?, IN(rrnD-rrnE)1, rpsL151(strR), polA12(ts), rha-5, deoC2?
KL405	13	F-, araC14, leuB6(Am), lacZ36, proC32, hisF860(Stable), thyA54, rpsE2115(SpcR), rpsL109(strR), malA38, xylA5, mtl-1, polA12(ts), thiE1
KL413	14	F-, araC14, leuB6(Am), lacZ36, proC32, hisF860(Stable), recA200(ts), thyA54, rpsE2115(SpcR), rpsL109(strR), malA38, xylA5, mtl-1, polA12(ts), thiE1
KL414	14	F-, araC14, leuB6(Am), lacZ36, proC32, λ^-, hisF860(Stable), recA56, thyA54, rpsE2115(SpcR), rpsL109(strR), xylA5, mtlA2, polA12(ts), thiE1