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Mutation polA1(Am)

• Name: polA1(Am)
• Type: Unknown
• Mutation of Gene: polA
• Approx. Map Location: 87.18
• Properties

  Property	Symbol
  amber(UAG) mutation	Am

  
  
• Comments
  • normal levels exonuc.;premature term.of polymerase <1% WT level. MMS^S,UV^S, viable at all temps. Grindley MGG143
• References
  • Grindley, ND, WS Kelley 1976. Effects of different alleles of the E. coli K12 pol A gene on the replication of non-transferring plasmids. Mol. Gen. Genet. 143(3):311-8.
• 22 Strains Carrying This Mutation

  Name	Mutations	Genotype
  H560	4	F+, tsx-79, endA2, rpsL8 or rpsL154(strR), polA1(Am)
  WP6	4	F-, sulA1?, trpE65(Oc), polA1(Am), [mal+]K-12(λS)
  WP67	4	F-, sulA1?, trpE65(Oc), polA1(Am), uvrA155
  P3478	5	F-, λ-, e14-, thyA36, IN(rrnD-rrnE)1, polA1(Am), deoC2
  CBK103	6	F-, λ-, thyA36, IN(rrnD-rrnE)1, cysG98::Tn5, polA1(Am), deoC2
  KNK402	6	F+, tsx-79, [gyrA43](ts), thyA755, endA2, rpsL8 or rpsL154(strR), polA1(Am)
  TA340	6	F-, ara-600, Δ(codB-lacI)3, zig-1863::Tn10, polA1(Am), thiE1?, Δ(purD-metA)768
  CM5409	7	F+, Δ(galK-bioD)76, λ-, relA1, spoT1, zig-1863::Tn10, polA1(Am), thiE1
  TA410	7	F-, ara-600, Δ(codB-lacI)3, zig-1863::Tn10, polA1(Am), Δ(rrsB-rrlB)769, thiE1?, Δ(purD-metA)768
  BT1026	8	F+, dnaE1026(ts), tsx-79, thyA55, endA2, rpsL8 or rpsL154(strR), polA1(Am), deo-38, φXs
  TA420	8	F-, ara-600, Δ(rrsH-rrlH)770, Δ(codB-lacI)3, zig-1863::Tn10, polA1(Am), Δ(rrsB-rrlB)769, thiE1?, Δ(purD-metA)768
  TA472	8	F-, ara-600, Δ(codB-lacI)3, zig-1863::Tn10, Δ(rrsA-rrlA)775::cat, polA1(Am), Δ(rrsB-rrlB)769, thiE1?, Δ(purD-metA)768
  JW164	9	F-, lacZ53(Am), λ-, thyA36, IN(rrnD-rrnE)1, rpsL151(strR), polA1(Am), rha-5, malB45, deoC2
  JW164	9	F-, lacZ53(Am), λ-, e14-, thyA36, rpsL151(strR), polA1(Am), rha-5, malB45, deoC2
  TA447	9	F-, ara-600, Δ(rrsH-rrlH)770, Δ(codB-lacI)3, Δ(rrlG-rrsG)772::lacZ, zig-1863::Tn10, polA1(Am), Δ(rrsB-rrlB)769, thiE1?, Δ(purD-metA)768
  HMS83	10	F-, polB100, lacZ53(Am), λ-, thyA36, IN(rrnD-rrnE)1, rpsL151(strR), polA1(Am), rha-5, deoC2?, lys-34
  NY73	10	F-, leuB6(Am), λ-, thyA47, dnaG3(ts), IN(rrnD-rrnE)1, rpsL153(strR), metE70, polA1(Am), rpoB316, deoC3?
  RK9117	10	F-, [araD139]B/r, Δ(argF-lac)169, non-9, gyrA219(NalR), relA1, rpsL150(strR), Δ(uhpT-ilvB)2095, zig-621::Tn10 uhpA+B+CconT+, polA1(Am), thiE1
  RK9118	10	F-, [araD139]B/r, Δ(argF-lac)169, non-9, gyrA219(NalR), relA1, rpsL150(strR), Δ(uhpT-ilvB)2095, zig-621::Tn10 uhpA+B+C-T+, polA1(Am), thiE1
  KL198	12	F-, galK35, λ-, pyrD34, trpC45, his-68, thyA53, rpsE2117(SpcR), rpsL118(strR), bgl-1, ilvD16, polA1(Am), deoC2 or deo-44
  KL407	14	F-, araC14, leuB6(Am), lacZ36, proC32, hisF860(Stable), recA200(ts), thyA54, rpsE2115(SpcR), rpsL109(strR), malA38, xylA5, mtl-1, polA1(Am), thiE1
  KL403	15	F-, araC14, leuB6(Am), lacZ36, proC32, λ-, sup-78(Mal+), hisF860(Stable), thyA54, rpsE2115(SpcR), rpsL109(strR), malA38, xylA5, mtl-1, polA1(Am), thiE1