E. coli Genetic Resources at Yale
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Mutation thyA12

• Name: thyA12
• Type: Unknown
• Mutation of Gene: thyA
• Approx. Map Location: 63.86
• Comments
  • Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• References
• 9 Strains Carrying This Mutation

  Name	Mutations	Genotype
  AB4046	15	F+, thr-1, araC14, leuB6(Am), Δ(gpt-proA)62, lacY1, tsx-33, galK2(Oc), hisG4(Oc), thyA6, thyA12, rpsL31(strR) or rpsL67(strR), xylA5, mtl-1, thiE1, dnaB43(ts)
  AB4031	16	F-, thr-1, araC14, leuB6(Am), Δ(gpt-proA)62, lacY1, tsx-33, glnX44(AS)?, galK2(Oc), hisG4(Oc), thyA12, rpsL31(strR), xylA5, mtl-1, thiE1, dnaB43(ts), drm-6
  AB6006	17	F-, thr-1, araC14, leuB6(Am), Δ(gpt-proA)62, lacY1, tsx-33, glnX44(AS)?, galK2(Oc), λ-, thyA12, rpsL31(strR), xylA5, mtl-1, argE3(Oc), thiE1, drm-6, [lig-7](ts)
  GM1	18	F-, thr-1, araC14, leuB6(Am), Δ(gpt-proA)62, lacY1, tsx-33, qsr'-0, glnX44(AS), galK2(Oc), λ-, hisG4(Oc), thyA12, rpsL31(strR), xylA5, mtl-1, metB1, thiE1, deoB16
  AB4027	19	F-, thr-1, araC14, leuB6(Am), secA205(aziR), Δ(gpt-proA)62, lacY1, tsx-33, qsr'-0, glnX44(AS), galK2(Oc), λ-, hisG4(Oc), thyA12, rpsL31(strR), xylA5, mtl-1, argE3(Oc), thiE1, drm-6
  GM18	19	F-, thr-1, araC14, leuB6(Am), Δ(gpt-proA)62, trmB1, lacY1, tsx-33, qsr'-0, glnX44(AS), galK2(Oc), λ-, hisG4(Oc), thyA12, rpsL31(strR), xylA5, mtl-1, metB1, thiE1, deoB16
  GM19	19	F-, thr-1, araC14, leuB6(Am), Δ(gpt-proA)62, lacY1, tsx-33, qsr'-0, glnX44(AS), galK2(Oc), λ-, hisG4(Oc), trmC2, thyA12, rpsL31(strR), xylA5, mtl-1, metB1, thiE1, deoB16
  AB2497	22	F-, thr-1, araC14, leuB6(Am), Δ(gpt-proA)62, lacY1, tsx-33, qsr'-0, glnX44(AS), galK2(Oc), λ-, Rac-0, hisG4(Oc), rfbC1, mgl-51, thyA12, rpsL31(strR), kdgK51, xylA5, mtl-1, argE3(Oc), thiE1, deoB16
  GM23	23	F-, thr-1, araC14, leuB6(Am), Δ(gpt-proA)62, lacY1, tsx-33, qsr'-0, glnX44(AS), galK2(Oc), λ-, Rac-0, hisG4(Oc), rfbC1, mgl-51, thyA12, rpsL31(strR), kdgK51, xylA5, mtl-1, metB1, trmA14, thiE1, deoB16