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Site pLC34-46B
- Name: pLC34-46B
- Type: Plasmid
- SubSites:
nadB trmC ranA ung pssA - Plasmid Sites:
araC14 galK2(Oc) galT22 glnX44(AS) lacY1 LAM- leuB6(Am) mtl-1 recA56 thiE1 thr-1 trpE63(del) xylA5 - Priority: 0
- Comment:
- Mixed clone. Hybridizes with Kohara clones 253-254, 435-437, 119, 146, and 450. See also pLC34-46A.
- References:
- Clarke, L, J Carbon 1976. A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome. Cell 9(1):91-9.
- Raetz, CR, TJ Larson, W Dowhan 1977. Gene cloning for the isolation of enzymes of membrane lipid synthesis: phosphatidylserine synthase overproduction in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 74(4):1412-6.
- Clarke, L., J. Carbon 1979. Selection of specific clones from colony banks by suppression or complementation tests. Methods Enzymol. 68:396-408
- Ohta, T., K. Waggoner, K. Louie, W. Dowhan 1981. Cloning of genes involved in membrane lipid synthesis. Effects of amplification of phosphatidylserine synthetase in Escherichia coli. J.Biol.Chem. 256:2219-2225
- Nishimura, A., K. Akiyama, Y. Kohara, K. Horiuchi 1992. Correlation of a subset of the pLC plasmids to the physical map of Escherichia coli K-12. Microbiol.Rev. 56:137-151
- 1 Strains Carrying This Plasmid
Name Mutations Genotype pLC34-46 13 F+, thr-1, araC14, leuB6(Am), lacY1, glnX44(AS), galK2(Oc), galT22, λ-, ΔtrpE63, recA56, xylA5, mtl-1, thiE1, pLC34-46B