Site trmE
- Name: trmE
- Type: Gene
- Synonyms
b3706 MG1655 locus tag mnmE thdF thiophene utilization - Mnemonic: tRNA methyltransferase
- Left End Point: 83.73
- Right End Point: 83.76
- Products:
GTPase involved in synthesis of hypermodified nucleoside, tRNA 5-methylaminomethyl-2-thiouridine - Direction: >
- Properties:
Property Comment member of GTPase family distinct from regulatory GTPases - Priority: 1
- 2 Alleles of This Gene
trmE737(del)::kan trmE792-YFP(::cat) - External Database Links:
Host Site Page Links EcoGene.org EG10997 EcoliWiki trmE - Comment:
- 5-methylaminoethyl-2-thiouridine synthesis; modifies some tRNA wobble positions. Sequencing showed that trmE and thdF (thiophene utilization) are the same. Essential in some backgrounds.
- References:
- Elseviers, D., L.A. Petrullo, P. Gallagher 1984. Novel E. coli mutants deficient in biosynthesis of 5-methylaminomethyl-2-thiouridine. Nucleic Acids Res. 12:3521-3533
- Alam, K.Y., D.P. Clark 1991. Molecular cloning and sequence of the thdF gene, which is involved in thiophene and furan oxidation by Escherichia coli. J.Bacteriol. 173:6018-6024
- Burland, V.D., G. Plunkett, D.L. Daniels, F.R. Blattner 1993. DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication. Genomics 16:551-561
- Cabedo, H., F. Macian, M. Villarroya, J.C. Escudero, M. Martinez-Vicente, E. Knecht, M.E. Armengod 1999. The Escherichia coli trmE (mnmE) gene, involved in tRNA modification, codes for an evolutionarily conserved GTPase with unusual biochemical propert