E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

About the CGSC

• CGSC Home
• Scope of the Collection
• The Database
• Privacy Policy
• Acknowledgment of Support

Searching the CGSC Database

• Help With Query Forms
• Strain Query
• Mutation Query
• Gene/Site Query
• Gene Product Query
• Reference Query
• Query Help
• Info on Genetic Nomenclature

How to Request Strains or Information

• Contact Information
• Charges
• Reviving Strains
• Strains Not Found?

Other CGSC Information

• FAQ on Procedures
• Current Working Map
• 1998 MMBR Map
• 1998 MMBR Gene List
• Map Diagrams (PDFs)

Other Links

• E. coli Links
• Other Stock Collections
• Other Bio Links
• Yale Web

Funded by a grant from the National Science Foundation

Site hupB

• Name: hupB
• Type: Gene
  Synonyms

  b0440	MG1655 locus tag
  dpeA	Manna and Gowrishankar
  hopD

  
  
• Mnemonic: HU-protein (histone-like)
• Left End Point: 9.93
• Right End Point: 9.94
  Products:

  HU-1, histone-like protein

  
  
• Direction: >
• Properties:

  Property	Comment
  transcription factor gene	member of type family, HU, IHF, and subtilis phage SPO1's TF1

  
  
• Priority: 1
  2 Alleles of This Gene

  hupB726(del)::kan

  hupB792-YFP(::cat)

  
  
• External Database Links:

  Host Site	Page Links
  NCBI/GenBank	X16540
  EcoGene.org	EG10467
  EcoliWiki	hupB

  
  
• Comment:
  • Histone-like protein HU-1 (HU-, NS1)
• References:
  • Laine, B., D. Kmiecik, P. Sautiere, G. Biserte, M. Cohen-Solal 1980. Complete amino-acid sequences of DNA-binding proteins HU-1 and HU-2 from Escherichia coli. Eur.J.Biochem. 103:447-461
  
  
  • Kano, Y., S. Yoshino, C. Wada, K. Yokoyama, M. Nobuhara, F. Imamoto 1985. Molecular cloning and nucleotide sequence of the HU-1 gene of Escherichia coli. Mol.Gen.Genet. 201:360-362
  
  
  • Kano, Y., C. Wada, T. Nagase, F. Imamoto 1986. Genetic characterization of the gene hupB encoding the HU-1 protein of Escherichia coli. Gene 45:37-44
  
  
  • Storts, D.R.X., A. Markovitz 1988. Construction and characterization of mutations in hupB, the gene encoding HU-beta-(HU-1) in Escherichia coli K-12. J.Bacteriol. 170:1541-1547
  
  
  • Kohno, K., M. Wada, Y. Kano, F. Imamoto 1990. Promoters and autogenous control of the Escherichia coli hupA and hupB genes. J.Mol.Biol. 213:27-36
  
  
  • Goshima, N., K. Kohno, F. Imamoto, Y. Kano 1990. HU-1 mutants of Escherichia coli deficient in DNA binding. Gene 96:141-145
  
  
  • Manna, D., J. Gowrishankar 1994. Evidence for involvement of proteins HU and RpoS in transcription of the osmoresponsive proU operon in Escherichia coli. J.Bacteriol. 176:5378-5384
  
  
  • Jaffe, A., D. Vinella, R. D'Ari 1997. The Escherichia coli histone-like protein HU affects DNA initiation, chromosome partitioning via MukB, and cell division via MinCDE. J.Bacteriol. 179:3494-3499
  
  
  • Polaczek, P., K. Kwan, J.L. Campbell 1998. Unwinding of the Escherichia coli origin of replication (oriC) can occur in the absence of initiation proteins but is stabilized by DnaA and histone-like proteins IHF or HU. Plasmid 39:77-83