E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Site recG

• Name: recG
• Type: Gene
  Synonyms

  b3652	MG1655 locus tag
  spoV?

  
  
• Mnemonic: Recombination
• Left End Point: 82.40
• Right End Point: 82.45
  Products:

  junction-specific DNA helicase

  
  
• Direction: >
• Priority: 1
  8 Alleles of This Gene

  recG162

  recG1909(del)::dhfr

  recG1919(del)::apra

  recG2102

  recG263(::kan)

  recG265(::cat)

  recG756(del)::kan

  recG794-YFP(::cat)

  
  
• External Database Links:

  Host Site	Page Links
  NCBI/GenBank	M64367
  EcoGene.org	EG10829
  EcoliWiki	recG

  
  
• Comment:
  • RecG catalyzes branch migration of Holliday junctions. See Ruv ABC also
• References:
  • Lloyd, R.G., G.J. Sharples 1991. Molecular organization and nucleotide sequence of the recG locus of Escherichia coli K-12. J.Bacteriol. 173:6837-6843
  
  
  • Lloyd, R.G., C. Buckman 1991. Genetic analysis of the recG locus of Escherichia coli K-12 and of its role in recombination and DNA repair. J.Bacteriol. 173:1004-1011
  
  
  • Lloyd, R.G. 1991. Conjugational recombination in resolvase-deficient ruvC mutants of Escherichia coli K-12 depends on recG. J.Bacteriol. 173:5414-5418
  
  
  • Kalman, M., H. Murphy, M. Cashel 1992. The nucleotide sequence of recG, the distal spo operon gene in Escherichia coli K-12. Gene 110:95-99
  
  
  • Lloyd, R.G., G.J. Sharples 1993. Dissociation of synthetic Holliday junctions by E. coli RecG protein. EMBO J. 12:17-22
  
  
  • Lloyd, R.G., G.J. Sharples 1993. Processing of recombination intermediates by the RecG and RuvAB proteins of Escherichia coli. Nucleic Acids Res. 21:1719-1725
  
  
  • Nagel, R., A. Chan, E. Rosen 1994. Ruv and recG genes and the induced precise excision of Tn10 in Escherichia coli. Mutat.Res. 311:103-109
  
  
  • Whitby, M.C., S.D. Vincent, R.G. Lloyd 1994. Branch migration of Holliday junctions: identification of RecG protein as a junction specific DNA helicase. EMBO J. 13:5220-5228
  
  
  • Foster, P.L., J.M. Trimarchi, R.A. Maurer 1996. Two enzymes, both of which process recombination intermediates, have opposite effects on adaptive mutation in Escherichia coli. Genetics 142:25-37
  
  
  • Al-Deib, A.A., A.A. Mahdi, R.G. Lloyd 1996. Modulation of recombination and DNA repair by the RecG and PriA helicases of Escherichia coli K-12. J.Bacteriol. 178:6782-6789
  
  
  • Zahradka, K., D. Zahradka, M. Petranovic 2001. Loss of lambda prophage recombinogenicity in UV-irradiated Escherichia coli: the role of host genes ruvA, ruvB, ruvC, and recG. Res.Microbiol. 152:873-881