E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Site recB

• Name: recB
• Type: Gene
  Synonyms

  b2820	MG1655 locus tag
  rorA

  
  
• Mnemonic: Recombination
• Left End Point: 63.60
• Right End Point: 63.68
  Products:

  RecBCD enzyme subunit

  exonuclease V subunit

  exonuclease/helicase

  
  
• Direction: <
• Priority: 1
  9 Alleles of This Gene

  recB1067(D1067A)

  recB1080(D1080A)

  recB1082(K1082Q)

  recB1910(del)::dhfr

  recB21

  recB270(ts)

  recB60

  recB745(del)::kan

  recB791-YFP(::cat)

  
  
• External Database Links:

  Host Site	Page Links
  NCBI/GenBank	X04581
  EcoGene.org	EG10824
  EcoliWiki	recB

  
  
• Comment:
  • Muts are def. for homologous recomb., sensitive to DNA=damaging agents and unable to restrict foreign DNA and produce inviable cells in culture. RecBCD req. for MMS repair, not for recomb.
• References:
  • Hickson, I.D., P.T. Emmerson 1981. Identification of the Escherichia coli recB and recC gene products. Nature 294:578-580
  
  
  • Sasaki, M., T. Fujiyoshi, K. Shimada, Y. Takagi 1982. Fine structure of the recB and recC gene region of Escherichia coli. Biochem.Biophys.Res.Commun. 109:414-422
  
  
  • Dykstra, C.C., D. Prasher, S.R. Kushner 1984. Physical and biochemical analysis of the cloned recB and recC genes of Escherichia coli K-12. J.Bacteriol. 157:21-27
  
  
  • Chaudhury, A.M., G.R. Smith 1985. Role of Escherichia coli RecBC enzyme in SOS induction. Mol.Gen.Genet. 201:525-528
  
  
  • Finch, P.W., A. Storey, K.E. Chapman, K. Brown, I.D. Hickson, P.T. Emmerson 1986. Complete nucleotide sequence of the Escherichia coli recB gene. Nucleic Acids Res. 14:8573-8582
  
  
  • Clark, A.J. 1991. rec genes and homologous recombination proteins in Escherichia coli. Biochimie 73:523-532
  
  
  • Estevenon, A.M., J. Kooistra, N. Sicard 1995. An Escherichia coli strain deficient for both exonuclease V and deoxycytidine triphosphate deaminase shows enhanced sensitivity to ionizing radiation. Mol.Gen.Genet. 246:514-518
  
  
  • Taylor, A.F., G.R. Smith 1995. Monomeric RecBCD enzyme binds and unwinds DNA. J.Biol.Chem. 270:24451-24458
  
  
  • Taylor, A.F., G.R. Smith 1995. Strand specificity of nicking of DNA at Chi sites by RecBCD enzyme. Modulation by ATP and magnesium levels. J.Biol.Chem. 270:24459-24467
  
  
  • Bianco, P.R., S.C. Kowalczykowski 1997. The recombination hotspot Chi is recognized by the translocating RecBCD enzyme as the single strand of DNA containing the sequence 5'-GCTGGTGG-3'. Proc.Natl.Acad.Sci.USA 94:6706-6711
  
  
  • Poteete, A.R., A.C. Fenton 2000. Genetic requirements of phage lambda red-mediated gene replacement in Escherichia coli K-12. J.Bacteriol. 182:2336-2340