E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Site ogt

• Name: ogt
• Type: Gene
  Synonyms

  b1335	MG1655 locus tag

  
  
• Mnemonic: O-methylguanine transferase
• Left End Point: 30.13
• Right End Point: 30.14
  Products:

  O6-methylguanine-DNA methyltransferase, constitutive

  
  
• Direction: <
• Properties:

  Property	Comment
  repair system gene	repairs alkylated DNA lesions

  
  
• Priority: 0
  2 Alleles of This Gene

  ogt-772(del)::kan

  ogt-791-YFP(::cat)

  
  
• External Database Links:

  Host Site	Page Links
  EcoGene.org	EG10668
  EcoliWiki	ogt

  
  
• Comment:
  • constitutively expressed
• References:
  • Potter, P.M., M.C. Wilkinson, J. Fitton, F.J. Carr, J. Brennand, D.P. Cooper, G.P. Margison 1987. Characterisation and nucleotide sequence of ogt, the O6-alkylguanine-DNA-alkyltransferase gene of E. coli. Nucleic Acids Res. 15:9177-9193
  
  
  • Potter, P.M., L.C. Harris, G.P. Margison 1989. Mapping of OGT in the E.coli chromosome. Nucleic Acids Res. 17:10505-0
  
  
  • Potter, P.M., K. Kleibl, L. Cawkwell, G.P. Margison 1989. Expression of the ogt gene in wild-type and ada mutants of E. coli. Nucleic Acids Res. 17:8047-8060
  
  
  • Margison, G.P., D.P. Cooper, P.M. Potter 1990. The E. coli ogt gene. Mutat.Res. 233:15-21
  
  
  • Rebeck, G.W., L.D. Samson 1991. Increased spontaneous mutation and alkylation sensitivity of Escherichia coli strains lacking the ogt O6-methylguanine DNA repair methyltransferase. J.Bacteriol. 173:2068-2076
  
  
  • Mackay, W.J., S. Han, L.D. Samson 1994. DNA alkylation repair limits spontaneous base substitution mutations in Escherichia coli. J.Bacteriol. 176:3224-3230
  
  
  • Abril, N., C. Hera, E. Alejandre, J.A. Rafferty, G.P. Margison, C. Pueyo 1994. Effect of ogt expression on mutation induction by methyl-, ethyl- and propylmethanesulphonate in Escherichia coli K12 strains. Mol.Gen.Genet. 242:744-748
  
  
  • Janion, C. 1995. Mechanisms of action of methyl methanesulfonate on Escherichia coli: mutagenesis, DNA damage and repair. Postepy Biochem. 41:308-313
  
  
  • Delaney, J.C., J.M. Essigmann 2001. Effect sequence context on O(6)-methylguanine repair and replication in vivo. Biochemistry 40:14968-14975