E. coli Genetic Resources at Yale
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Site ispH

• Name: ispH
• Type: Gene
  Synonyms

  b0029	MG1655 locus tag
  lytB	early observations of phiX174 lysis and autolytic response to antibiotics

  
  
• Mnemonic: isoprenoid synthesis
• Left End Point: 0.57
• Right End Point: 0.59
  Products:

  regulatory protein for RelA

  hydroxy-methyl-butenyl-diP reductase

  1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase

  
  
• Direction: >
• Properties:

  Property	Comment
  regulatory gene	regulates RelA (pGp synthetase)
  essential gene	NULL

  
  
• Priority: 0
  2 Alleles of This Gene

  ispH3(del)::kan

  ispH795-YFP(::cat)

  
  
• External Database Links:

  Host Site	Page Links
  EcoGene.org	EG11081
  EcoliWiki	ispH

  
  
• Comment:
  • isoprenoid (terpene) synthesis. Also reported to affect penicillin tolerance and control of stringent response and regulate RelA
• References:
  • Lubitz, W., R.E. Harkness, E.E. Ishiguro 1984. Requirement for a functional host cell autolytic enzyme system for lysis of Escherichia coli by bacteriophage phiX174. J.Bacteriol. 159:385-387
  
  
  • Kusser, W., E.E. Ishiguro 1987. Suppression of mutations conferring penicillin tolerance by interference with the stringent control mechanism of Escherichia coli. J.Bacteriol. 169:4396-4398
  
  
  • Bouvier, J., P. Stragier 1991. Nucleotide sequence of the lsp-dapB interval in Escherichia coli. Nucleic Acids Res. 19:180
  
  
  • Harkness, R.E., W. Kusser, B.J. Qi, E.E. Ishiguro 1992. Genetic mapping of the lytA and lytB loci of Escherichia coli, which are involved in penicillin tolerance and control of the stringent response. Can.J.Microbiol. 38:975-978
  
  
  • Gustafson, C.E., S. Kaul, E.E. Ishiguro 1993. Identification of the Escherichia coli lytB Gene, which is involved in penicillin tolerance and control of the stringent response. J.Bacteriol. 175:1203-1205
  
  
  • Potter, S., X. Yang, M.J. Boulanger, E.E. Ishiguro 1998. Occurrence of homologs of the Escherichia coli lytB gene in gram-negative bacterial species. J.Bacteriol. 180:1959-1961
  
  
  • McAteer, S., A. Coulson, N.F. McLennan, M. Masters 2001. The lytB gene of Escherichia coli is essential and specifies a product needed for isoprenoid biosynthesis. J.Bacteriol. 183:7403-7407
  
  
  • Adam, P., S. Hecht, W. Eisenreich, J. Kaiser, T. Grawert, D. Arigoni, A. Bacher, F. Rohdich 2002. Biosynthesis of terpenes: studies on 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase. Proc.Natl.Acad.Sci.USA 99:12108-12113
  
  
  • Wolff, M., M. Seemann, B. Tse Sum Bui, Y. Frapart, D. Tritsch, A.G. Estrabot, M. Rodriguez-Concepcion, A. Boronat, A. Marquet, M. Rohmer 2003. Isoprenoid biosynthesis via the methylerythritol phosphate pathway: the (E)-4-hydroxy-3-methylbut-2-enyl diph
  
  
  • Rohdich, F., F. Zepeck, P. Adam, S. Hecht, J. Kaiser, R. Laupitz, T. Grawert, S. Amslinger, W. Eisenreich, A. Bacher, D. Arigoni 2003. The deoxyxylulose phosphate pathway of isoprenoid biosynthesis: studies on the mechanisms of the reactions catalyzed