Site phoA
- Name: phoA
- Type: Gene
- Synonyms
b0383 MG1655 locus tag - Mnemonic: Phosphate
- Left End Point: 8.64
- Right End Point: 8.67
- Products:
alkaline phosphatase - Direction: >
- Priority: 1
- External Database Links:
Host Site Page Links NCBI/GenBank M33966 NCBI/GenBank M13345 EcoGene.org EG10727 EcoliWiki phoA - Comment:
- Selectable phenotypes. Const.: growth on beta-glycerolPO4 + high PO4. In upp-,phoS-,or phoT-: loss gives 5-FU + Ad resist. In glpD-: B-glyceroPO4 resist. Indicator plates, naphthoazol dyes.(Vinopal)
- References:
- Torriani, A., F. Rothman 1961. Mutants of Escherichia coli constitutive for alkaline phosphatase. J.Bacteriol. 81:835-836
- Messer, W., W. Vielmetter 1965. High resolution colony staining for the detection of bacterial growth requirement mutants using naphthol azodye techniques. Biochem.Biophys.Res.Commun. 21:182-186
- Wanner, B.L., P. Latterell 1980. Mutants affected in alkaline phosphatase expression: evidence for multiple positive regulators of the phosphate regulon in Escherichia coli. Genetics 96:353-366
- Inouye, M., S. Michaelis, A. Wright, J.R. Beckwith 1981. Cloning and restriction mapping of the alkaline phosphatase structural gene (phoA) of Escherichia coli and generation of deletion mutants in vitro. J.Bacteriol. 146:668-675
- Sarthy, A., S. Michaelis, J.R. Beckwith 1981. Deletion map of the Escherichia coli structural gene for alkaline phosphatase, phoA. J.Bacteriol. 145:288-292
- Berg, P.E. 1981. Cloning and characterization of the Escherichia coli gene coding for alkaline phosphatase. J.Bacteriol. 146:660-667
- Wanner, B.L., S. Wieder, R. McSharry 1981. Use of bacteriophage transposon Mu d1 to determine the orientation for three proC-linked phosphate-starvation-inducible (psi) genes in Escherichia coli K-12. J.Bacteriol. 146:93-101
- Sarthy, A., S. Michaelis, J.R. Beckwith 1981. Use of gene fusions to determine the orientation of gene phoA on the Escherichia coli chromosome. J.Bacteriol. 145:293-298
- Kikuchi, Y., K. Yoda, M. Yamasaki, G. Tamura 1981. The nucleotide sequence of the promoter and the amino-terminal region of alkaline phosphatase structural gene (phoA) of Escherichia coli. Nucleic Acids Res. 9:5671-5678
- Bradshaw, R.A., F. Cancedda, L.H. Ericsson, P.A. Neumann, S.P. Piccoli, M.J. Schlesinger, K. Shriefer, K.A. Walsh 1981. Amino acid sequence of Escherichia coli alkaline phosphatase. Proc.Natl.Acad.Sci.USA 78:3473-3477
- Inouye, M., W. Barnes, J.R. Beckwith 1982. Signal sequence of alkaline phosphatase of Escherichia coli. J.Bacteriol. 149:434-439
- Boidol, W., M. Simonis, M. Topert, G. Siewert 1982. Recombinant plasmids with genes for the biosynthesis of alkaline phosphatase of Escherichia coli. Mol.Gen.Genet. 185:510-512
- Heyde, M., R. Portalier 1982. New pleiotropic alkaline phosphatase-negative mutants of Escherichia coli K-12. J.Bacteriol. 151:529-533
- Hadley, R.G., M. Hu, M. Timmons, K. Yun, R. Deonier 1983. A partial restriction map of the proA-purE region of the E. coli K-12 chromosome. Gene 22:281-287
- Shuttleworth, H., J. Taylor, N.P. Minton 1986. Sequence of the gene for alkaline phosphatase from Escherichia coli JM83. Nucleic Acids Res. 14:8689
- Chang, C.N., W.-J. Kuang, E.Y. Chen 1986. Nucleotide sequence of the alkaline phosphatase gene of Escherichia coli. Gene 44:121-125
- Vinopal, R. 1987. E. coli and Salmonella, Neidhardt et al., ed., 2:990
- Freimuth, P.I., J.W. Taylor, E.T. Kaiser 1990. Introduction of guest peptides into Escherichia coli alkaline phosphatase. Excision and purification of a dynorphin analogue from an active chimeric protein. J.Biol.Chem. 265:896-901
- Xu, X., E.R. Kantrowitz 1992. The importance of Aspartate 327 for catalysis and zinc binding in Escherichia coli alkaline phosphatase. J.Biol.Chem. 267:16244-16251
- Kamitani, S., Y. Akiyama, K. Ito 1992. Identification and characterization of an Escherichia coli gene required for the formation of correctly folded alkaline phosphatase, a periplasmic enzyme. EMBO J. 11:57-62
- Muller, BH, C Lamoure, MH Le Du, L Cattolico, E Lajeunesse, F Lemaître, A Pearson, F Ducancel, A Ménez, JC Boulain 2001. Improving Escherichia coli alkaline phosphatase efficacy by additional mutations inside and outside the catalytic pocket. Chembiochem 2(7-8):517-23.