Site lacY
- Name: lacY
- Type: Gene
- Synonyms
b0343 MG1655 locus tag - Mnemonic: Lactose
- Left End Point: 7.79
- Right End Point: 7.81
- Products:
galactoside permease (M protein) Lac permease - Direction: <
- Properties:
Property Comment transport gene symporter cotransports H+ and lactose - Priority: 1
- 10 Alleles of This Gene
lacY0(Am) lacY1 lacY29 lacY328(Am) lacY40 lacY41 lacY784(del)::kan lacY83 lacY884(del)::FRT lacY96 - External Database Links:
Host Site Page Links EcoGene.org EG10526 EcoliWiki lacY - Comment:
- Selectable phenotype. Loss (alone or w/lacZ pol.mut.):0-nitro- phenylthiogalactoside res.;phenyl- or phenethylgalactoside res. or res. to sim. cmpds.; growth on L-arabinose + isopropylgalac.
- References:
- Horiuchi, T., S. Horiuchi, A. Novick 1963. The genetic basis of hyper-synthesis of beta-galactoside. Genetics 48:157-169
- Muller-Hill, B., L. Crapo, W. Gilbert 1968. Mutants that make more lac repressor. Proc.Natl.Acad.Sci.USA 59:1259-1264
- Johnston, M.A., H. Pivnick 1970. Use of autocytotoxic beta-D-galactosides for selective growth of Salmonella typhimurium in the presence of coliforms. J.Microbiol.Serol. 16:83-89
- Smith, T.F., J.R. Sadler 1971. The nature of lactose operator constitutive mutations. J.Mol.Biol. 59:273-305
- Buchel, D.E., B. Gronenborn, B. Muller-Hill 1980. Sequence of the lactose permease gene. Nature 283:541-545
- Wandersman, C., F. Moreno, M. Schwartz 1980. Pleiotropic mutations rendering Escherichia coli K-12 resistant to bacteriophage TP1. J.Bacteriol. 143:1374-1383
- Ehring, R., K. Beyreuther, J.K. Wright, P. Overath 1980. In vitro and in vivo products of E. coli lactose permease gene are identical. Nature 283:537-540
- Bibi, E., H.R. Kaback 1992. Functional complementation of internal deletion mutants in the lactose permease of Escherichia coli. Proc.Natl.Acad.Sci.USA 89:1524-1528
- Olsen, S.G., K.M. Greene, R.J. Brooker 1993. Lactose permease mutants which transport malto-oligosaccharides. J.Bacteriol. 175:6269-6275
- Pogliano, J.A., J.R. Beckwith 1994. SecD and SecF facilitate protein export in Escherichia coli. EMBO J. 13:554-561
- He, M.M., J. Sun, H.R. Kaback 1996. Cysteine-scanning mutagenesis of transmembrane domain XII and the flanking periplasmic loop in the lactose permease of Escherichia coli. Biochemistry 35:12909-12914
- Blumenthal, R.M., D.W. Borst, R.G. Matthews 1996. Experimental analysis of global gene regulation in Escherichia coli. Prog. Nucleic Acid Res. Mol. Biol. 55:1-86
- Bogdanov, M., M. Umeda, W. Dowhan 1999. Phospholipid-assisted refolding of an integral membrane protein.Minimum structural features for phosphatidylethanolamine to act as a molecular chaperone. J.Biol.Chem. 274:12339-12345
- Guan, L., F.D. Murphy, H.R. Kaback 2002. Surface-exposed positions in the transmembrane helices of the lactose permease of Escherichia coli determined by intermolecular thiol cross-linking. Proc.Natl.Acad.Sci.USA 99:3475-3480
- Wada, R., S. Matsuyama, H. Tokuda 2004. Targeted mutagenesis of five conserved tryptophan residues of LolB involved in membrane localization of Escherichia coli lipoproteins. Biochem.Biophys.Res.Commun. 323:1069-1074
- Vazquez-Ibar, J.L., L. Guan, A.B. Weinglass, G. Verner, R. Gordillo, H.R. Kaback 2004. Sugar recognition by the lactose permease of Escherichia coli. J.Biol.Chem. 279:49214-49221