E. coli Genetic Resources at Yale
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Site exbB

• Name: exbB
• Type: Gene
  Synonyms

  b3006	MG1655 locus tag

  
  
• Mnemonic: export
• Left End Point: 67.88
• Right End Point: 67.90
  Products:

  membrane protein required for TonB-dependent energy transduction and TonB stability

  TonB-ExbB-ExbD cytoplasmic membrane-anchored complex component

  
  
• Direction: <
• Properties:

  Property	Comment
  encodes membrane protein	TonB-ExbB-ExbD complex transfers energy to open channels in OMP receptors; couples action of OM transporters FhuA and BtuB across cytopl.memb.

  
  
• Priority: 1
  2 Alleles of This Gene

  exbB41

  exbB779(del)::kan

  
  
• External Database Links:

  Host Site	Page Links
  EcoGene.org	EG10271
  EcoliWiki	exbB

  
  
• Comment:
  • Uptake of enterochelin; resistance or sensitivity to colicins. Selectable phenotype. Colicin B, colicin I, other colicin resistance. Strong homology to TolQ (and exbD homol. tolR)
• References:
  • Guterman, S.K., L. Dann 1973. Excretion of enterochelin by exbA and exbB mutants of Escherichia coli. J.Bacteriol. 114:1225-1230
  
  
  • Pugsley, A.P., P.R. Reeves 1976. Iron uptake in colicin B-resistant mutants of Escherichia coli K-12. J.Bacteriol. 126:1052-1062
  
  
  • Eick-Helmerich, K., K. Hantke, V. Braun 1987. Cloning and expression of the exbB gene of Escherichia coli K-12. Mol.Gen.Genet. 206:246-251
  
  
  • Fischer, E., K. Gunter, V. Braun 1989. Involvement of ExbB and TonB in transport across the outer membrane of Escherichia coli: phenotypic complementation of exb mutants by overexpressed tonB and physical stabilization of TonB by ExbB. J.Bacteriol. 171
  
  
  • Eick-Helmerich, K., V. Braun 1989. Import of biopolymers into Escherichia coli: nucleotide sequences of the exbB and exbD genes are homologous to those of the tolQ and tolR genes, respectively. J.Bacteriol. 171:5117-5126
  
  
  • Braun, V. 1989. The structurally related exbB and tolQ genes are interchangeable in conferring tonB-dependent colicin, bacteriophage, and albomycin sensitivity. J.Bacteriol. 171:6387-6390
  
  
  • Braun, V., C. Herrmann 1993. Evolutionary relationship of uptake systems for biopolymers in Escherichia coli: cross-complementation between the TonB-ExbB-ExbD and the TolA-TolQ-TolR proteins. Mol.Microbiol. 8:261-268
  
  
  • Karlsson, M., K. Hannavy, C.F. Higgins 1993. ExbB acts as a chaperone-like protein to stabilize TonB in the cytoplasm. Mol.Microbiol. 8:389-396
  
  
  • Kampfenkel, K., V. Braun 1993. Topology of the ExbB protein in the cytoplasmic membrane of Escherichia coli. J.Biol.Chem. 268:6050-6057
  
  
  • Killmann, H., V. Braun 1994. Energy-dependent receptor activities of Escherichia coli K-12: mutated TonB proteins alter FhuA receptor activities to phages T5, T1, phi80 and to colicin M. FEMS Microbiol.Lett. 119:71-76
  
  
  • Ahmer, B.M., M.G. Thomas, R. Larsen, K. Postle 1995. Characterization of the exbBD operon of Escherichia coli and the role of ExbB and ExbD in TonB function and stability. J.Bacteriol. 177:4742-4747