E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Site endA

• Name: endA
• Type: Gene
  Synonyms

  b2945	MG1655 locus tag

  
  
• Mnemonic: endonuclease
• Left End Point: 66.57
• Right End Point: 66.59
  Products:

  DNA-specific endonuclease I

  
  
• Direction: >
• Priority: 1
  11 Alleles of This Gene

  endA1

  endA101

  endA2

  endA3

  endA4

  endA5

  endA6(del-ins)::kan

  endA7(del-ins)::Cm

  endA720(del)::kan

  endA8(del:Frt:Tc)::Tc

  endA9(del-ins)::FRT

  
  
• External Database Links:

  Host Site	Page Links
  EcoGene.org	EG11336
  EcoliWiki	endA

  
  
• Comment:
  • select for mut. by methyl green (or Giemsa) staining for undegraded DNA. Endo I respons. for extensive DNA brkdown. endA- mutants improve miniprep dsDNA.
• References:
  • Wright, M. 1971. Mutants of Escherichia coli lacking endonuclease I, ribonuclease I or ribonuclease II. J.Bacteriol. 107:87-94
  
  
  • Jekel, M., W. Wackernagel 1994. Location of the endA gene coding for endonuclease I on the physical map of the Escherichia coli K-12 chromosome. J.Bacteriol. 176:1550-1551