E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Site cysG

• Name: cysG
• Type: Gene
  Synonyms

  b3368	MG1655 locus tag

  
  
• Mnemonic: Cysteine
• Left End Point: 75.35
• Right End Point: 75.38
  Products:

  uroporphyrinogen III methyltransferase

  siroheme synthetase

  ferrochelatase and oxidase

  S-adenosylmethionine uroporphyrinogen III methylase

  
  
• Direction: >
• Priority: 1
  7 Alleles of This Gene

  cysG132

  cysG303

  cysG44

  cysG49

  cysG55

  cysG776(del)::kan

  cysG98::Tn5

  
  
• External Database Links:

  Host Site	Page Links
  NCBI/GenBank	X14202
  EcoGene.org	EG10188
  EcoliWiki	cysG

  
  
• Comment:
  • Transcribed from nirB operon promoter and also from a cysG promoter
• References:
  • Cole, J.A., B.M. Newman, P. White 1980. Biochemical and genetic characterization of nirB mutants of Escherichia coli K12 pleiotropically defective in nitrite and sulphite reduction. J.Gen.Microbiol. 120:475-483
  
  
  • Jackson, G.S., A. Cornish-Bowden, J.A. Cole 1981. Prosthetic groups of the NADH-dependent nitrite reductase from Escherichia coli K12. Biochem.J. 193:861-867
  
  
  • MacDonald, H., N.R. Pope, J.A. Cole 1985. Isolation, characterization and complementation analysis of nirB mutants of Escherichia coli deficient only in NADH-dependent nitrite reductase activity. J.Gen.Microbiol. 131:2771-2782
  
  
  • MacDonald, H., J.A. Cole 1985. Molecular cloning and functional analysis of the cysG and nirB genes of Escherichia coli K12, two closely-linked genes required for NADH-dependent nitrite reductase activity. Mol.Gen.Genet. 200:328-334
  
  
  • Tei, H., K. Murata, A. Kimura 1990. Molecular cloning of the cys genes (cysC, cysD, cysH, cysI, cysJ, and cysG) responsible for cysteine biosynthesis in Escherichia coli K-12. Biotechnol.Appl.Biochem. 12:212-216
  
  
  • Peakman, T.C., S.J. Busby, J.A. Cole 1990. Transcriptional control of the cysG gene of Escherichia coli K-12 during aerobic and anaerobic growth. Eur.J.Biochem. 191:325-331
  
  
  • Peakman, T.C., J. Crouzet, J.-F. Mayaux, S.J. Busby, S. Mohan, N. Harborne, J.C. Wootton, R. Nicolson, J.A. Cole 1990. Nucleotide sequence, organisation and structural analysis of the products of genes in the nirB-cysG region of the Escherichia coli K-
  
  
  • Harborne, N., L. Griffiths, S.J. Busby, J.A. Cole 1992. Transcriptional control, translation and function of the products of the five open reading frames of the Escherichia coli nir operon. Mol.Microbiol. 6:2805-2813
  
  
  • Spencer, J.B., N.J. Stolowich, C.A. Roessner, A.I. Scott 1993. The Escherichia coli cysG gene encodes the multifunctional protein, siroheme synthase. FEBS Lett. 335:57-60
  
  
  • Woodcock, S.C., E. Raux, F. Levillayer, C. Thermes, A. Rambach, M.J. Warren 1998. Effect of mutations in the transmethylase and dehydrogenase/chelatase domains of sirohaem synthase (CysG) on sirohaem and cobalamin biosynthesis. Biochem.J. 330:121-129