E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

About the CGSC

• CGSC Home
• Scope of the Collection
• The Database
• Privacy Policy
• Acknowledgment of Support

Searching the CGSC Database

• Help With Query Forms
• Strain Query
• Mutation Query
• Gene/Site Query
• Gene Product Query
• Reference Query
• Query Help
• Info on Genetic Nomenclature

How to Request Strains or Information

• Contact Information
• Charges
• Reviving Strains
• Strains Not Found?

Other CGSC Information

• FAQ on Procedures
• Current Working Map
• 1998 MMBR Map
• 1998 MMBR Gene List
• Map Diagrams (PDFs)

Other Links

• E. coli Links
• Other Stock Collections
• Other Bio Links
• Yale Web

Funded by a grant from the National Science Foundation

Site hinT

• Name: hinT
• Type: Gene
  Synonyms

  b1103	MG1655 locus tag
  ycfF

  
  
• Mnemonic: HIstidine triad nucleotide-binding (rabbit protein
• Left End Point: 25.03
• Right End Point: 25.03
  Products:

  purine nucleoside phosphoramidase (guanosine-preferring)

  Hint (His-triad nucleotide binding proteing) homolog

  
  
• Direction: >
• Priority: 0
  2 Alleles of This Gene

  hinT765(del)::kan

  hinT791-YFP(::cat)

  
  
• External Database Links:

  Host Site	Page Links
  EcoGene.org	EG12172
  EcoliWiki	hinT

  
  
• Comment:
  • cation-resistance. Mutants fail to grow in media with, e.g., 0.9 M NaCl, 0.75 M KCl.
• References:
  • Chou, T.F., P. Bieganowski, K. Shilinski, J. Cheng, C. Brenner, C.R. Wagner 2005. 31P NMR and genetic analysis establish hinT as the only Escherchia coli purine nucleoside phosphoramidase and as essential for growth under high salt conditions. J.Biol.C