Strain - F500/GMS724

Strain - F500/GMS724Strain F500/GMS724

Strain F500/GMS724

CGSC#: 5505
Strain Designation
Designation Source Preference
F500/GMS724 Novel G. 1
Sex(Hfr,F+,F-,or F'): F'

11 Mutations:

Mutation	Location	Certainty	Display
lacY1	7.79	1	lacY1
tsx-29	9.28	0	tsx-29
glnX44(AS)	14.99	0	glnX44(AS)
galK2(Oc)	16.99	1	galK2(Oc)
LAM-	17.40	1	λ^-
manA4	36.35	1	manA4
aroD6	38.21	1	aroD6
recA1	60.80	1	recA1
rpsL700(strR)	74.84	1	rpsL700(strR)
mtl-1	81.26	1	mtl-1
metB1	88.94	1	metB1

Comments:
- This strain must be maintained on minimal medium with mannose as carbon source, containing Met and lacking aromatic amino acids.
Episome/Plasmid: F500
Plasmid Markers/Mutations:
References:
- Novel, M., G. Novel 1976. Regulation of beta-glucuronidase synthesis in Escherichia coli K-12: constitutive mutants specifically derepressed for uidA expression. J.Bacteriol. 127:406-417
Printable Strain Report