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Strain χ2338

• CGSC#: 7036
• Strain Designation

  Designation	Source	Preference
  χ2338	Curtiss R.	1
  chi2338		2

  
  
• Sex(Hfr,F+,F-,or F'): F-
• 17 Mutations:

  Mutation	Location	Certainty	Display
  araC766(del)	1.52	1	ΔaraC766
  fhuA53	3.61	1	fhuA53
  dapD8	3.99	1	dapD8
  gltA16	16.22	1	gltA16
  DE(gal-modC)690	16.87	1	Δ(gal-modC)690
  LAM-	17.40	1	λ-
  minB-2	26.37	1	minB-2
  DE(trpB-trpC)513	28.34	1	Δ(trpB-trpC)513
  rfbC1	45.43	1	rfbC1
  gyrA25(NalR)	50.33	1	gyrA25(NalR)
  thyA57(del)	63.86	1	ΔthyA57
  endA1	66.57	1	endA1
  aroB15	75.77	1	aroB15
  cycA1	95.44	1	cycA1
  hsdR2	98.74	1	hsdR2
  cycB2	1000.00	1	cycB2
  oms-1	1000.00	1	oms-1

  
  
• Comments:
  • The rfbD mutation in this strain was called rfb-2, but is presumably the same as rfbD1
  • W. Jacobs points out that this strain should be grown up and frozen in glycerol. It does not survive well on Luria agar at 4 degrees C.
• References:
  • Jacobs, WR, JF Barrett, JE Clark-Curtiss, R Curtiss 1986. In vivo repackaging of recombinant cosmid molecules for analyses of Salmonella typhimurium, Streptococcus mutans, and mycobacterial genomic libraries. Infect. Immun. 52(1):101-9.
  
  

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