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Strain pLC3-13/JA200

• CGSC#: 6678
• Strain Designation

  Designation	Source	Preference
  pLC3-13/JA200		1

  
  
• Sex(Hfr,F+,F-,or F'): F+
• 13 Mutations:

  Mutation	Location	Certainty	Display
  thr-1	0.01	1	thr-1
  araC14	1.52	1	araC14
  leuB6(Am)	1.74	1	leuB6(Am)
  lacY1	7.79	1	lacY1
  glnX44(AS)	14.99	1	glnX44(AS)
  galK2(Oc)	16.99	1	galK2(Oc)
  galT22	17.01	1	galT22
  LAM-	17.40	1	λ-
  trpE63(del)	28.44	1	ΔtrpE63
  recA56	60.80	1	recA56
  xylA5	80.34	1	xylA5
  mtl-1	81.26	1	mtl-1
  thiE1	90.34	1	thiE1

  
  
• Comments:
  • The Clarke and Carbon plasmid pLC3-13 carries dnaJ, rpsT, lspA and does ont carry ileS or dapB.
• Episome/Plasmid:

  Plasmid Markers/Mutations:
  araC14
  galK2(Oc)
  galT22
  glnX44(AS)
  lacY1
  LAM-
  leuB6(Am)
  mtl-1
  recA56
  thiE1
  thr-1
  trpE63(del)
  xylA5
  dnaJ
  rpsT
  lspA

• References:
  • Tokunaga, M., J.M. Loranger, H.C. Wu 1983. Isolation and characterization of an Escherichia coli clone overproducing prolipoprotein signal peptidase. J.Biol.Chem. 258:12102-12105
  • Innis, M.A., M. Tokunaga, M.E. Williams, J.M. Loranger, S.-Y. Chang, S. Chang, H.C. Wu 1984. Nucleotide sequence of the Escherichia coli prolipoprotein signal peptidase (lsp) gene. Proc.Natl.Acad.Sci.USA 81:3708-3712
  
  

  Printable Strain Report