Strain - DG31

Strain - DG31Strain DG31

Strain DG31

Designation	Source	Preference
DG31	Gelfand D.H.	1

21 Mutations:

Mutation	Location	Certainty	Display
DE(gpt-proA)62	5.51	1	Δ(gpt-proA)62
lacY1	7.79	1	lacY1
tsx-33	9.28	1	tsx-33
glnX44(AS)	14.99	1	glnX44(AS)
galK2(Oc)	16.99	1	galK2(Oc)
LAM-	17.40	1	λ^-
Rac-0	30.39	1	Rac-0
sbcB15	44.85	1	sbcB15
hisG4(Oc)	45.01	1	hisG4(Oc)
rfbC1	45.43	1	rfbC1
recB21	63.60	1	recB21
recC22	63.74	1	recC22
rpsL31(strR)	74.84	1	rpsL31(strR)
kdgK51	79.26	1	kdgK51
xylA5	80.34	1	xylA5
mtl-1	81.26	1	mtl-1
ilvE12	85.15	1	ilvE12
argE3(Oc)	89.48	1	argE3(Oc)
thiE1	90.34	1	thiE1
hsdS14	98.67	1	hsdS14
hppT-29	1000.00	1	hppT-29

Comments:
- 1.Note on tyrB^- and aspC^- strains:
- 2.For growth of these strains, rich media should be supplemented with tyrosine and phenylalanine (50 ug/ml).
- 3.Minimal medium should be supplemented with asparagine, glutamic acid and glutamine (100 ug/ml), succinate (2.5 mg/ml), malate (2.5 mg/ml) and a-ketoglutarate (1 mg/ml).
- 4.For a discussion of the problems encountered in using these markers in transduction studies, see Gelfand & Rudo 1977 J. Bacteriol. 130:441.
References:
- Gelfand, DH, RA Steinberg 1977. Escherichia coli mutants deficient in the aspartate and aromatic amino acid aminotransferases. J. Bacteriol. 130(1):429-40.
- Gelfand, D.H., N. Rudo 1977. Mapping of the aspartate and aromatic amino acid amino-transferase genes tyrB and aspC. J.Bacteriol. 130:441-444
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