E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Strain TOP10/pPFINT

• CGSC#: 12374
• Strain Designation

  Designation	Source	Preference
  TOP10/pPFINT	Romeo T.	1
  pPFINT		2

  
  
• Sex(Hfr,F+,F-,or F'): F-
• 12 Mutations:

  Mutation	Location	Certainty	Display
  DE(araA-leu)7697	1.35	1	Δ(araA-leu)7697
  araD139([B/r])	1.42	1	[araD139]B/r
  DE(codB-lacI)3	7.60	1	Δ(codB-lacI)3
  phi80dlacZ58(M15)	8.00	1	φ80dlacZ58(M15)
  galK0	16.99	1	galK0
  mcrA0	26.07	1	mcrA0
  galU-	27.82	1	galU-
  recA1	60.80	1	recA1
  endA1	66.57	1	endA1
  nupG-	66.90	1	nupG-
  rpsL-(strR)	74.84	1	rpsL-(strR)
  DE(mcrC-mrr)715	98.59	1	Δ(mcrC-mrr)715

  
  
• Comments:
  • The pFINT plasmid is essentially a tetracycline resistant version of pINT-ts
• Episome/Plasmid: pPFINT

  Plasmid Markers/Mutations:

• References:
  • Edwards, AN, LM Patterson-Fortin, CA Vakulskas, JW Mercante, K Potrykus, D Vinella, MI Camacho, JA Fields, SA Thompson, D Georgellis, M Cashel, P Babitzke, T Romeo 2011. Circuitry linking the Csr and stringent response global regulatory systems. Mol. Microbiol. 80(6):1561-80.
  
  

  Printable Strain Report