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Strain AB2481

• CGSC#: 2481
• Strain Designation

  Designation	Source	Preference
  AB2481	Howard-Flanders P.	1

  
  
• Sex(Hfr,F+,F-,or F'): ?
• 14 Mutations:

  Mutation	Location	Certainty	Display
  thr-1	0.01	1	thr-1
  araC14	1.52	0	araC14
  leuB6(Am)	1.74	1	leuB6(Am)
  DE(gpt-proA)62	5.51	1	Δ(gpt-proA)62
  lacY1 or lacZ4	8.00	0	lacY1 or lacZ4
  tsx-33	9.28	0	tsx-33
  galK2(Oc)	16.99	0	galK2(Oc)
  LAM-	17.40	0	λ-
  recA14	60.80	1	recA14
  rpsL31(strR) or rpsL8	73.00	1	rpsL31(strR) or rpsL8
  xylA5	80.34	0	xylA5
  mtl-1	81.26	0	mtl-1
  thiE1	90.34	1	thiE1
  uvrA6	92.01	1	uvrA6

  
  
• Comments:
  • In Lyophil Only. SEE NOTE re VIABILITY
  • This strain is sensitive to male-specific phage MS2, but mates and acts as an acceptor strain with Fgal episomes, so it may be a defective male.
  • This strain may carry an amber suppressor.
• References:
  • Howard-Flanders, P, L Theriot, JB Stedeford 1969. Some properties of excision-defective recombination-deficient mutants of Escherichia coli K-12. J. Bacteriol. 97(3):1134-41.
  
  

  Printable Strain Report