Strain - BL21(DE3)/pLysS

Strain - BL21(DE3)/pLysSStrain BL21(DE3)/pLysS

Strain BL21(DE3)/pLysS

Designation	Source	Preference
BL21(DE3)/pLysS		1

7 Mutations:

Mutation	Location	Certainty	Display
lon-11	9.88	1	lon-11
DE(ompT-nfrA)885	12.59	1	Δ(ompT-nfrA)885
DE(galM-ybhJ)884	16.96	1	Δ(galM-ybhJ)884
LAMDE3	17.38	1	λDE3 [lacI, lacUV5-T7 gene 1, ind1, sam7, nin5]
DE46	46.69	1	Δ46
mal+([K-12])(LamS)	91.40	1	[mal⁺]_K-12(λ^S)
hsdS10	98.67	1	hsdS10

Comments:
- Not a K-12 strain. E. coli B derivative.
- This is a descendant of E.coli B.Bc, cured of a defective prophage found in the wildtype (Δ46). The malK, lamB, malM region was transduced in from E.coli K-12.
Episome/Plasmid: pLysS
Plasmid Markers/Mutations:
ori p15A
T7 lysozyme
cat
References:
- Wood, W.B. 1966. Host specificity of DNA produced by Escherichia coli: bacterial mutations affecting the restriction and modification of DNA. J.Mol.Biol. 16:118-133
Printable Strain Report