Strain Ra-2
- CGSC#: 4241
- Strain Designation
Designation Source Preference Ra-2 Cappello M.C. 1 CSH60 3 - Sex(Hfr,F+,F-,or F'): Hfr
- Hfr Point of Origin#: 48
- PO Map Position: 88.40
- Direction of Marker Transfer: Clockwise
- 4 Mutations:
Mutation Location Certainty Display glnV42(AS) 14.99 1 glnV42(AS) LAM- 17.40 1 λ- Sfa(att)4 65.00 1 Sfa(att)4 malB28(LamR) 92.00 1 malB28(λR) - Comments:
- Ra-2 is a rather unstable Hfr, switching between Hfr Ra-2 and Hfr Ra-1. sfa-4 is a sex factor attachment site at the point of origin of Ra-1.
- References:
- Joset, F., B. Low, R. Krisch 1964. Induction by radiation of a new direction of chromosome transfer during conjugation in an Hfr strain of Escherichia coli. Biochem.Biophys.Res.Commun. 17:742-747
- Low, K.B. 1967. Inversion of transfer modes and sex factor-chromosome interactions in conjugation in Escherichia coli. J.Bacteriol. 93:98-106
- Low, K.B. 1968. Formation of merodiploids in matings with a class of Rec- recipient strains of Escherichia coli K12. Proc.Natl.Acad.Sci.USA 60:160-167
- Low, K.B. 1973. Rapid mapping of conditional and auxotrophic mutants of Escherichia coli K-12. J.Bacteriol. 113:798-812
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