E. coli Genetic Resources at Yale
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Strain GBE180

• CGSC#: 7569
• Strain Designation

  Designation	Source	Preference
  GBE180	Barcak G.J.	1
  DH5alpha,pcnB-		2

  
  
• Sex(Hfr,F+,F-,or F'): F-
• 12 Mutations:

  Mutation	Location	Certainty	Display
  pcnB80	3.40	1	pcnB80
  zad-2084::Tn10	3.40	1	zad-2084::Tn10
  DE(argF-lac)169	6.21	1	Δ(argF-lac)169
  phi80dlacZ58(M15)	8.00	1	φ80dlacZ58(M15)
  glnX44(AS)	14.99	1	glnX44(AS)
  LAM-	17.40	1	λ-
  rfbC1	45.43	1	rfbC1
  gyrA96(NalR)	50.33	1	gyrA96(NalR)
  recA1	60.80	1	recA1
  spoT1	82.34	1	spoT1
  thiE1	90.34	1	thiE1
  hsdR17	98.74	1	hsdR17

  
  
• Comments:
• References:
  • Pierson, V.L., G.J. Barcak 1999. Development of E. coli host strains tolerating unstable DNA sequences on ColE1 vectors. Focus 21:18-19
  
  

  Printable Strain Report