E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Strain PK3

• CGSC#: 5862
• Strain Designation

  Designation	Source	Preference
  PK3	Kahn P.	1

  
  
• Sex(Hfr,F+,F-,or F'): Hfr
• Hfr Point of Origin#: 131(ColV.)
• PO Map Position: 80.13
• Direction of Marker Transfer: Clockwise
• 9 Mutations:

  Mutation	Location	Certainty	Display
  thr-1	0.01	1	thr-1
  leuB6(Am)	1.74	1	leuB6(Am)
  secA215	2.33	1	secA215
  fhuA21	3.61	1	fhuA21
  lacY1	7.79	1	lacY1
  glnX44(AS)	14.99	1	glnX44(AS)
  LAM-	17.40	1	λ-
  rfbC1	45.43	1	rfbC1
  thiE1	90.34	1	thiE1

  
  
• Comments:
  • This is a ColV Hfr. It is ColE1 Resistant, and ColV Resistant and probably ColV⁺.
• References:
  • KAHN, P, DR HELINSKI 1964. RELATIONSHIP BETWEEN COLICINOGENIC FACTORS E1 AND V AND AN F FACTOR IN ESCHERICHIA COLI. J. Bacteriol. 88:1573-9.
  • Kahn, PL 1968. Isolation of high-frequency recombining strains from Escherichia coli containing the V colicinogenic factor. J. Bacteriol. 96(1):205-14.
  
  

  Printable Strain Report